The animals realize that Horton was right all along, thinking that the Kangaroo was trying to crush Horton's spirit and build her own reputation in the jungle. The Kangaroo's son, Rudy, grabs the flower and warns everyone that the whos are here. At the last minute, JoJo grabs the horn used to project Horton's voice, runs up the highest tower and yells 'YOPP!', breaking through the sound barrier, just before the speck hits the oil. The Kangaroo takes the clover and drops it into the boiling oil. The Mayor enlists all of his people to make noise by shouting 'We are here,' as well as playing a variety of instruments, so the animals may hear them. When Horton refuses, she orders the animals to rope and cage him and issue Whoville's destruction via a pot of boiling beezelnut oil. The Kangaroo offers Horton to give up the speck. After a failed attempt by Vlad, the Kangaroo finds out that Horton still has the speck, and decides to rally the jungle community into confronting Horton by telling lies that Horton's goal will lead to anarchy. The Kangaroo eventually enlists a vulture named Vlad Vladikoff (Will Arnett) to get rid of the speck by force.

Also taking force toward Horton are the Wickersham brothers, a group of bullying monkeys who love making misery. Jane Kangaroo (Carol Burnett), not believing Horton's beliefs, tries to get Horton to give up the speck, but Horton refuses. LaRue that Whoville will be destroyed if Horton does not find a 'safer, more stable home.' Horton resolves to place the speck atop Mt. Despite being the oldest and next in line for the mayoral position, JoJo doesn't want to be mayor, and he's so scared of disappointing his father, he rarely ever speaks.The Mayor finds out from Dr. He has a wife, Sally, 96 daughters (whose names all begin with the letter H), and one teenage son named JoJo. Horton finds out the speck harbors the city of Whoville and its inhabitants, led by Mayor Ned McDodd (Steve Carell). Believing that an entire family of microscopic creatures are living on that speck, he places it atop a clover. The speck of dust floats past him in the air, and he hears a tiny yelp coming from it. Meanwhile, a caring, imaginative elephant named Horton, the jungle's nature teacher, takes a dip in the pool.

In the Jungle of Nool, a tiny speck of dust has landed on a pink flower.

Hears a who